The 18 kDa translocator protein (TSPO) is used as a biomarker of neuroinflammation, but it may also play a direct role in the pathophysiology of neurological conditions with an inflammatory component such as Alzheimer’s disease. Here, we used functional ultrasound imaging (fUSi) to assess how TSPO knockout influences brain activity and connectivity in the 3xTgAD mouse model of AD.